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Displaying 1-9 of 9 results for: glycan

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Label-Free Profiling of mAb O-linked Glycans by HPLC with Charged Aerosol Detection

Instrument Type: HPLC-CAD

O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>

UHPLC Analysis of 2-Aminobenzamide-Labeled Glycans with the Vanquish Flex System

Instrument Type: HPLC (Biocompatible)

HPLC of 2-aminobenzamide (2-AB) labeled glycans with fluorescence detection is one of the major analyses in biotherapeutics characterization. Here we show the separation of 2-AB-glycans originated by bovine fetuin and human IgG with The Thermo Scientific Vanquish Flex UHPLC. The Thermo Scientific GlycanPac AXR-1 column is used for the fetuin glycans. The Thermo Scientific Accucore 150-Amide-HILIC is used for the IgG glycans.

High resolution analysis of 2-aminobenzamide labeled dextran ladder on a Thermo Scientific Accucore 150-Amide-HILIC nanoLC column

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific nanoLC system is applied for the analysis of 2-aminobenzamide labeled dextran ladder. The separation is performed on a Thermo Scientific Accucore 150-Amide-HILIC nanoLC column, with UV detection at 240 and 330 nm.

Evaluation of temperature influence on retention of glycans in HILIC

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex Ultimate 3000 BioRS system in combination with the Thermo Scientific Accucore 150-Amide-HILIC is used to run separation of 2-AB-labelled dextran ladder in gradient mode. The column compartment was set at different temperature between 20 and 50 °C. At high temperature the selectivity between oligomers was improved, and peaks were in average narrower. Consequently higher peak capacity was achieved at high temperature.

Glycans by UHPLC-CAD on Thermo Scientific Dionex UltiMate 3000 RSLC

Instrument Type: HPLC-CAD

Glycans released from bovine fetuin by PNGase-F are separated by gradient elution on the Thermo Scientific Dionex GlycanPac AXR-1 column and detected by charged aerosol detection on the Corona Veo RS.

Fast analysis of sugar phosphates by HPLC-CAD

Instrument Type: HPLC-CAD

The Thermo Scientific UltiMate 3000RS system is applied for the analysis of sugar phosphates. The separation is performed using a Thermo Scientific GlycanPac AXH-1 1.9µm column, with charged aerosol detection. The Thermo Scientific GlycanPac AXH-1 is a column designed to classify anionic carbohydrates by charge and resolve them by HILIC interactions. For this group of simple sugar phosphates, an isocratic analysis is sufficient using a volatile mobile phase that is compatible with charged aerosol detection or mass spectrometry.

Excellent separation of human IgG glycans on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column

Instrument Type: HPLC

Human IgG glycans were separated on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column using 2-AB derivatization and fluorescence detection.

A simple HILIC method for the analysis of bovine fetuin glycans using a Thermo Scientific Accucore 150-Amide-HILIC HPLC column

Instrument Type: HPLC

The analysis of 2AB labelled bovine fetuin glycans was performed on the Thermo Scientific Accucore 150-Amide-HILIC HPLC column. The analysis was simple and robust, leading to the separation and detection of eight individual glycan peaks.

Enhanced separation of a 2-aminobenzamide labelled dextran ladder using a Thermo Scientific Accucore 150-Amide-HILIC column

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of a 2-aminobenzamide labelled dextran ladder. The separation is performed on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column, with fluorescence detection.