High-sensitivity low-nano flow LC-MS methods for high-throughput sample-limited proteomics
Instrument Type: nanoLCMS
We developed five high-sensitivity, high-throughput nano-LC-MS methods that afford industry-leading protein coverage for label-free DDA and DIA. With a 250 pg HeLa digest in a 10-min LC gradient, it identifies:
• ~800 protein groups in DDA (SEQUEST & INFERYS)
• >1,800 protein groups in DDA (CHIMERYS)
• >3,000 protein groups in DIA (SN17)
These methods support SCP analysis with up to 72 cells per day (CPD) in LFQ-DDA & DIA modes.