Thermo Scientific AppsLab Library: High-sensitivity low-nano flow LC-MS methods for high-throughput sample-limited proteomics

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nanolcms methods for singlecell proteomics

Description

We developed five high-sensitivity, high-throughput nano-LC-MS methods that afford industry-leading protein coverage for label-free DDA and DIA. With a 250 pg HeLa digest in a 10-min LC gradient, it identifies: • ~800 protein groups in DDA (SEQUEST & INFERYS) • >1,800 protein groups in DDA (CHIMERYS) • >3,000 protein groups in DIA (SN17) These methods support SCP analysis with up to 72 cells per day (CPD) in LFQ-DDA & DIA modes.

Market:	Proteomics
Keywords:	direct injection, SCP, Orbitrap Exploris 480, bottom-up proteomics, HeLa protein digest, Vanquish Neo UHPLC system, Acclaim PepMap 100 C18, data-dependent acquisition (DDA), data-independent acquisition (DIA), label-free quantification (LFQ), nano-LC-MS, sample-limited, single-cell proteomics, wide window acquisition (WWA)
Author:	Runsheng Zheng, Alec Valenta, Christopher Pynn, Ece Aydin, Wim Decrop, Martin Samonig

Uploaded on 3/17/2023.

For Research Use Only. Not for use in diagnostic procedures.